II-1. Artificially induced maturation and technique for induced spawning
نویسندگان
چکیده
منابع مشابه
Spawning, in vitro Maturation, and Changes in Oocyte Electrophysiology Induced by Serotonin in Tivela stultorum.
Spawning was induced in both male and female Pismo clams by injecting 0.4 ml of 5 mM serotonin into the gonad. Spawned oocytes had already matured to metaphase I of meiosis or were undergoing germinal vesicle breakdown at the time of release. Prophase-arrested oocytes scraped from the ovaries of uninjected clams were induced to undergo germinal vesicle breakdown in vitro by incubating them with...
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ment and increased linearly for 72 h. Receptor expression was dependent on new protein synthesis. At 48 h, Scatchard analysis demonstrated 2.4 X 105 receptors/ cell with a KD of 1.3 nM. In contrast to induction of HL60 differentiation by dimethyl sulfoxide, retinoic acid, or phorbol myristate acetate, the developmental programs initiated by agents that raised intracellular adenosine 3'5'-cyclic...
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ژورنال
عنوان ژورنال: NIPPON SUISAN GAKKAISHI
سال: 2014
ISSN: 0021-5392,1349-998X
DOI: 10.2331/suisan.80.994